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col2a antibody  (Huabio Inc)

 
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    Structured Review

    Huabio Inc col2a antibody
    Col2a Antibody, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/col2a+antibody/col2a/pm39107784-133-31-33
    Average 90 stars, based on 1 article reviews
    col2a antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Pulsed electromagnetic fields potentiate bone marrow mesenchymal stem cell chondrogenesis by regulating the Wnt/β-catenin signaling pathway.
    Article Snippet: Sections for immunofluorescence (IF) were processed as described in the IHC assay and then incubated with the following primary antibodies overnight to evaluate protein expression levels: ACAN (1:200, Novusbio Cat# NB600-504), COL2A (1:100, Huabio Cat# ET1611-56) and SOX9 (1:200, Huabio Cat# ET1611-56).

    Immunohistochemistry:

    Article Title: Pulsed electromagnetic fields potentiate bone marrow mesenchymal stem cell chondrogenesis by regulating the Wnt/β-catenin signaling pathway.
    Article Snippet: Sections for immunofluorescence (IF) were processed as described in the IHC assay and then incubated with the following primary antibodies overnight to evaluate protein expression levels: ACAN (1:200, Novusbio Cat# NB600-504), COL2A (1:100, Huabio Cat# ET1611-56) and SOX9 (1:200, Huabio Cat# ET1611-56).

    Incubation:

    Article Title: Pulsed electromagnetic fields potentiate bone marrow mesenchymal stem cell chondrogenesis by regulating the Wnt/β-catenin signaling pathway.
    Article Snippet: Sections for immunofluorescence (IF) were processed as described in the IHC assay and then incubated with the following primary antibodies overnight to evaluate protein expression levels: ACAN (1:200, Novusbio Cat# NB600-504), COL2A (1:100, Huabio Cat# ET1611-56) and SOX9 (1:200, Huabio Cat# ET1611-56).

    Expressing:

    Article Title: Pulsed electromagnetic fields potentiate bone marrow mesenchymal stem cell chondrogenesis by regulating the Wnt/β-catenin signaling pathway.
    Article Snippet: Sections for immunofluorescence (IF) were processed as described in the IHC assay and then incubated with the following primary antibodies overnight to evaluate protein expression levels: ACAN (1:200, Novusbio Cat# NB600-504), COL2A (1:100, Huabio Cat# ET1611-56) and SOX9 (1:200, Huabio Cat# ET1611-56).



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    CyA promotes chondrogenic differentiation of MSC . ( A ) Relative mRNA expression levels of ACAN and <t>COL2A</t> in MSC. ( B ) Alcian blue staining images of MSC cultured with CyA for 21 days. ( C ) Cell viability of MSC over 21 days. ( D ) Relative mRNA expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( E ) Protein expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( F ) Representative immunocytochemistry images of COL2A in MSC from Day 0 to Day 21. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.
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    Image Search Results


    CyA promotes chondrogenic differentiation of MSC . ( A ) Relative mRNA expression levels of ACAN and COL2A in MSC. ( B ) Alcian blue staining images of MSC cultured with CyA for 21 days. ( C ) Cell viability of MSC over 21 days. ( D ) Relative mRNA expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( E ) Protein expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( F ) Representative immunocytochemistry images of COL2A in MSC from Day 0 to Day 21. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.

    Journal: Aging and Disease

    Article Title: Enhanced Chondrogenic Potential and Osteoarthritis Treatment Using Cyaonoside A-Induced MSC Delivered via a Hyaluronic Acid-Based Hydrogel System

    doi: 10.14336/AD.2024.10016

    Figure Lengend Snippet: CyA promotes chondrogenic differentiation of MSC . ( A ) Relative mRNA expression levels of ACAN and COL2A in MSC. ( B ) Alcian blue staining images of MSC cultured with CyA for 21 days. ( C ) Cell viability of MSC over 21 days. ( D ) Relative mRNA expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( E ) Protein expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( F ) Representative immunocytochemistry images of COL2A in MSC from Day 0 to Day 21. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.

    Article Snippet: The COL2A primary antibody (1:1000, Cat# 28459-1-AP, Proteintech, USA) was applied, and the sections were incubated overnight at 4°C.

    Techniques: Expressing, Staining, Cell Culture, Immunocytochemistry

    Expression of ACAN, COL2A, SOX9, and MMP13 in chondrocytes . ( A ) Western blot analysis of ACAN, COL2A, SOX9, and MMP13 expression in chondrocytes. ( B ) Relative mRNA expression levels of ACAN, COL2A, SOX9, and MMP13 in chondrocytes. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). **P < 0.01, ***P < 0.001, and ns = no statistically significant difference between groups. ( C ) Alcian blue staining images of chondrocytes. ( D ) Immunofluorescence images of COL2A in chondrocytes. ( E ) Immunofluorescence images of MMP13 in chondrocytes.

    Journal: Aging and Disease

    Article Title: Enhanced Chondrogenic Potential and Osteoarthritis Treatment Using Cyaonoside A-Induced MSC Delivered via a Hyaluronic Acid-Based Hydrogel System

    doi: 10.14336/AD.2024.10016

    Figure Lengend Snippet: Expression of ACAN, COL2A, SOX9, and MMP13 in chondrocytes . ( A ) Western blot analysis of ACAN, COL2A, SOX9, and MMP13 expression in chondrocytes. ( B ) Relative mRNA expression levels of ACAN, COL2A, SOX9, and MMP13 in chondrocytes. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). **P < 0.01, ***P < 0.001, and ns = no statistically significant difference between groups. ( C ) Alcian blue staining images of chondrocytes. ( D ) Immunofluorescence images of COL2A in chondrocytes. ( E ) Immunofluorescence images of MMP13 in chondrocytes.

    Article Snippet: The COL2A primary antibody (1:1000, Cat# 28459-1-AP, Proteintech, USA) was applied, and the sections were incubated overnight at 4°C.

    Techniques: Expressing, Western Blot, Staining, Immunofluorescence

    In vivo therapeutic evaluation of hydrogel loaded with CyA-induced MSC . ( A ) Representative 3D reconstruction images of joints in different groups. ( B ) H&E staining of articular cartilage in different groups. ( C ) S&F staining of articular cartilage in different groups. ( D ) Immunohistochemical staining of COL2A in articular cartilage across different groups. ( E ) Immunofluorescence staining of Caspase-3 in articular cartilage across different groups. (F, G) Relative mRNA expression levels of ACAN and COL2A in joint tissues of different groups. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.

    Journal: Aging and Disease

    Article Title: Enhanced Chondrogenic Potential and Osteoarthritis Treatment Using Cyaonoside A-Induced MSC Delivered via a Hyaluronic Acid-Based Hydrogel System

    doi: 10.14336/AD.2024.10016

    Figure Lengend Snippet: In vivo therapeutic evaluation of hydrogel loaded with CyA-induced MSC . ( A ) Representative 3D reconstruction images of joints in different groups. ( B ) H&E staining of articular cartilage in different groups. ( C ) S&F staining of articular cartilage in different groups. ( D ) Immunohistochemical staining of COL2A in articular cartilage across different groups. ( E ) Immunofluorescence staining of Caspase-3 in articular cartilage across different groups. (F, G) Relative mRNA expression levels of ACAN and COL2A in joint tissues of different groups. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.

    Article Snippet: The COL2A primary antibody (1:1000, Cat# 28459-1-AP, Proteintech, USA) was applied, and the sections were incubated overnight at 4°C.

    Techniques: In Vivo, Staining, Immunohistochemical staining, Immunofluorescence, Expressing

    CyA promotes chondrogenic differentiation of MSC . ( A ) Relative mRNA expression levels of ACAN and COL2A in MSC. ( B ) Alcian blue staining images of MSC cultured with CyA for 21 days. ( C ) Cell viability of MSC over 21 days. ( D ) Relative mRNA expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( E ) Protein expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( F ) Representative immunocytochemistry images of COL2A in MSC from Day 0 to Day 21. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.

    Journal: Aging and Disease

    Article Title: Enhanced Chondrogenic Potential and Osteoarthritis Treatment Using Cyaonoside A-Induced MSC Delivered via a Hyaluronic Acid-Based Hydrogel System

    doi: 10.14336/AD.2024.10016

    Figure Lengend Snippet: CyA promotes chondrogenic differentiation of MSC . ( A ) Relative mRNA expression levels of ACAN and COL2A in MSC. ( B ) Alcian blue staining images of MSC cultured with CyA for 21 days. ( C ) Cell viability of MSC over 21 days. ( D ) Relative mRNA expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( E ) Protein expression levels of ACAN, COL2A, and SOX9 in MSC over 21 days. ( F ) Representative immunocytochemistry images of COL2A in MSC from Day 0 to Day 21. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.

    Article Snippet: Overnight incubation at 4°C with diluted primary antibodies followed: ACAN (1:2000, Cat# 68350-1-Ig, Proteintech, USA), COL2A (1:2000, Cat# 28459-1-AP, Proteintech, USA), SOX9 (1:2000, Cat# 67439-1-Ig, Proteintech, USA), MMP13 (1:2000, Cat# 18165-1-AP, Proteintech, USA), CD9 (1:1000, Cat# 13174, CST, USA), CD63 (1:1000, Cat# 52090, CST, USA), GM130 (1:1000, Cat# 12480, CST, USA), Bcl-2 (1:1000, Cat# 3498, CST, USA), P53 (1:1000, Cat# 9282, CST, USA), and GAPDH (1:10000, Cat# 10494-1-AP, Proteintech, USA).

    Techniques: Expressing, Staining, Cell Culture, Immunocytochemistry

    Expression of ACAN, COL2A, SOX9, and MMP13 in chondrocytes . ( A ) Western blot analysis of ACAN, COL2A, SOX9, and MMP13 expression in chondrocytes. ( B ) Relative mRNA expression levels of ACAN, COL2A, SOX9, and MMP13 in chondrocytes. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). **P < 0.01, ***P < 0.001, and ns = no statistically significant difference between groups. ( C ) Alcian blue staining images of chondrocytes. ( D ) Immunofluorescence images of COL2A in chondrocytes. ( E ) Immunofluorescence images of MMP13 in chondrocytes.

    Journal: Aging and Disease

    Article Title: Enhanced Chondrogenic Potential and Osteoarthritis Treatment Using Cyaonoside A-Induced MSC Delivered via a Hyaluronic Acid-Based Hydrogel System

    doi: 10.14336/AD.2024.10016

    Figure Lengend Snippet: Expression of ACAN, COL2A, SOX9, and MMP13 in chondrocytes . ( A ) Western blot analysis of ACAN, COL2A, SOX9, and MMP13 expression in chondrocytes. ( B ) Relative mRNA expression levels of ACAN, COL2A, SOX9, and MMP13 in chondrocytes. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). **P < 0.01, ***P < 0.001, and ns = no statistically significant difference between groups. ( C ) Alcian blue staining images of chondrocytes. ( D ) Immunofluorescence images of COL2A in chondrocytes. ( E ) Immunofluorescence images of MMP13 in chondrocytes.

    Article Snippet: Overnight incubation at 4°C with diluted primary antibodies followed: ACAN (1:2000, Cat# 68350-1-Ig, Proteintech, USA), COL2A (1:2000, Cat# 28459-1-AP, Proteintech, USA), SOX9 (1:2000, Cat# 67439-1-Ig, Proteintech, USA), MMP13 (1:2000, Cat# 18165-1-AP, Proteintech, USA), CD9 (1:1000, Cat# 13174, CST, USA), CD63 (1:1000, Cat# 52090, CST, USA), GM130 (1:1000, Cat# 12480, CST, USA), Bcl-2 (1:1000, Cat# 3498, CST, USA), P53 (1:1000, Cat# 9282, CST, USA), and GAPDH (1:10000, Cat# 10494-1-AP, Proteintech, USA).

    Techniques: Expressing, Western Blot, Staining, Immunofluorescence

    In vivo therapeutic evaluation of hydrogel loaded with CyA-induced MSC . ( A ) Representative 3D reconstruction images of joints in different groups. ( B ) H&E staining of articular cartilage in different groups. ( C ) S&F staining of articular cartilage in different groups. ( D ) Immunohistochemical staining of COL2A in articular cartilage across different groups. ( E ) Immunofluorescence staining of Caspase-3 in articular cartilage across different groups. (F, G) Relative mRNA expression levels of ACAN and COL2A in joint tissues of different groups. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.

    Journal: Aging and Disease

    Article Title: Enhanced Chondrogenic Potential and Osteoarthritis Treatment Using Cyaonoside A-Induced MSC Delivered via a Hyaluronic Acid-Based Hydrogel System

    doi: 10.14336/AD.2024.10016

    Figure Lengend Snippet: In vivo therapeutic evaluation of hydrogel loaded with CyA-induced MSC . ( A ) Representative 3D reconstruction images of joints in different groups. ( B ) H&E staining of articular cartilage in different groups. ( C ) S&F staining of articular cartilage in different groups. ( D ) Immunohistochemical staining of COL2A in articular cartilage across different groups. ( E ) Immunofluorescence staining of Caspase-3 in articular cartilage across different groups. (F, G) Relative mRNA expression levels of ACAN and COL2A in joint tissues of different groups. Statistical significance was determined using one-way ANOVA for multiple group comparisons, followed by post hoc tests. All data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, and ns = no statistically significant difference between groups.

    Article Snippet: Overnight incubation at 4°C with diluted primary antibodies followed: ACAN (1:2000, Cat# 68350-1-Ig, Proteintech, USA), COL2A (1:2000, Cat# 28459-1-AP, Proteintech, USA), SOX9 (1:2000, Cat# 67439-1-Ig, Proteintech, USA), MMP13 (1:2000, Cat# 18165-1-AP, Proteintech, USA), CD9 (1:1000, Cat# 13174, CST, USA), CD63 (1:1000, Cat# 52090, CST, USA), GM130 (1:1000, Cat# 12480, CST, USA), Bcl-2 (1:1000, Cat# 3498, CST, USA), P53 (1:1000, Cat# 9282, CST, USA), and GAPDH (1:10000, Cat# 10494-1-AP, Proteintech, USA).

    Techniques: In Vivo, Staining, Immunohistochemical staining, Immunofluorescence, Expressing